Protein Labeling Reagents
Sartorius pHrodo Orange Cell Labeling Kit, Real-Time Phagocytosis Quantification, Compatible With IncuCyte System, 1 Kit
The Sartorius IncuCyte pHrodo Orange Cell Labeling Kit provides a reliable solution for quantifying phagocytosis and efferocytosis using live-cell imaging. It features a unique pH-sensitive fluorophore that offers low fluorescence at neutral pH and a significant increase in fluorescence under acidic conditions. This enables real-time evaluation of cellular behaviors affected by various factors, setting it apart from other assays.
Cell Biolabs Inc OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP Sites), 50 assays
Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. The OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites) uses an Aldehyde Reactive Probe (ARP) to react specifically with an aldehyde group on the open ring form of AP sites. This allows for the AP sites to be tagged with biotin which is later detected with Streptavidin-Enzyme conjugate. The quantity of AP sites in unknown DNA sample is determined by comparing its absorbance with a standard curve generated from the provided DNA standard containing predetermined AP sites. The kit has a detection sensitivity range of 4 to 40 AP sites per 1 x 105 bp. Each kit provides sufficient reagents to perform up to 50 assays for unknown samples, excluding the standard curve.
Revvity Health Sciences Inc IVISense 680 NHS Fluorescent Dye (1 mg) (VivoTag)
IVISense 680 NHS Fluorescent Dye (1 mg) (VivoTag)
Revvity Health Sciences Inc IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)
IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)
Revvity Health Sciences Inc IVISense 680 Fluorescent Cell Labeling Dye (5 x 0.2mg) (VivoTrack)
IVISense 680 Fluorescent Cell Labeling Dye (5 x 0.2mg) (VivoTrack)
Revvity Health Sciences Inc IVISense 680 NHS Fluorescent Labeling Kit (VivoTag)
IVISense 680 NHS Fluorescent Labeling Kit (VivoTag)
Santa Cruz Biotechnology Chromeo™ P503 | 1mg
Chromeo™ P503 detects proteins and peptides by exhibiting a color change from blue to red. After binding to primary amines, it undergos a large shortwave spectral shift of more than 100nm that is visible to the naked eye. There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. The shift of the absorption & emission bands together with the increased fluorescence quantum yield effectively eliminates background from any unbound dye. In addition, because unbound the pyrylium dye is hydrolyzed during the labeling procedure, there is no need for purification following the simple one-step, room-temperature incubation. The labeled protein is ready to use immediately, and any possible background from the unbound dye is eliminated. Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point.
Biocare Medical, LLC Reveal Decloaker, 10X - 500 ml
Reveal Decloaker is a heat retrieval solution that is suitable for most antibody-antigen staining and reduces non-specific background staining, blocks endogenous peroxidase and removes mercury crystals. Reveal Decloaker can be used with Biocare's digital electric pressure cooker (Decloaking Chamber), a steamer, a water bath, or a microwave oven. For many antibodies, titers are doubled and background staining is significantly reduced when compared to citrate buffer, pH 6.0. Reveal Decloaker incorporates Assure technology, a color-coded high temperature pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Reveal Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.
Dojindo Molecular Technologies Inc PlasMem Bright Green dye (100 ul) offers plasma membrane staining for 24 hrs+ with clear visualization, high retentivity, low toxicity, water-soluble, no washing, applicable to live and fixed cells.
The plasma membrane consists of a lipid bilayer separating the intracellular environment from the extracellular space. Thus, the PM plays a central role in many cell behaviors such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to cell status and is linked to many diseases. PlasMem Bright dyes have higher retentivity on the plasma membrane. Furthermore, PlasMem Bright dyes are more water-soluble compared with other commercially available dyes and can be diluted with the culture medium. The PlasMem Bright dyes offer two different color options (green and red) and provide ready-to-use DMSO solutions. A working solution can be prepared easily via a single dilution step using growth medium or HBSS. Dojindo offers various reagents for cell membrane research. (https://www.dojindo.com/track-the-dynamics-of-plasma-membrane/)
Sino Biological Recombinant Human Complement C5 Protein (His Tag), HPLC-verified, Endotoxin-Free
A DNA sequence encoding the Human C5 (NP_001726.2) (Gln19-Cys1676) was expressed with a polyhistidine tag at the C-terminus.
Cayman Chemical AcrIdInIum NHS ester 1mg
The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS ester will produce chemiluminescence in the presence of hydrogen peroxide. Acridinium-labeled proteins can be used as a detection method in immunoassays.{2437,2438}